Yehia Daaka, PhD
Professor
Teaching Profile
Courses Taught
2018
GMS7980 Research for Doctoral Dissertation
2018
GMS7979 Advanced Research
2015-2016,2018
GMS6009 Principles of Drug Action and Therapeutics
2014,2016-2021,2021-2024
GMS6421 Cell Biology
2017
GMS6001 Fundamentals of Biomedical Sciences I
2013-2015
GMS6690 Molecular Cell Biology Journal Club
2013-2014
GMS6692 Research Conference in Anatomy and Cell Biology
2021-2024
GMS6647 Transcriptional and Translational Control of Cell Growth and Proliferation
2022,2024
GMS6064 Tumor Biology
2022-2024
GMS6691 Special Topics in Cell Biology and Anatomy
2023-2024
DEN5121 Biochm Molec Cell Bio
Research Profile
Research in the Daaka lab focuses on understanding the biological roles of G protein-coupled and androgen receptors that instruct embryonic development and continue to function in adulthood. He has been involved in crucial discoveries that unraveled contribution of these receptors to human pathophysiologic processes; for example, in the initiation and progression of cancer. Research from the Daaka lab on the function of these receptor systems has contributed to their recognition as targets for cancer therapy, and over the last decade several anti-cancer drugs have been developed against these molecules. Current projects examine contribution of the G protein-coupled receptors and their heterotrimeric G proteins and Arrestin effectors to the progression of prostate cancer from androgen-dependent to castration-resistant state, and in the metastasis of kidney cancer. We are also studying post-translational modification of androgen receptor by S-nitrosylation and its role in progression of the disease. Another area of investigation in the Daaka lab is centered on studying mechanisms involved in vesicle trafficking in recurrent urinary tract infections, with emphasis on the G proteins dynamin that support fission of vesicles from the plasma membrane into the cytosol.
Open Researcher and Contributor ID (ORCID)
0000-0002-9129-6593
Publications
2023
DAXX drives de novo lipogenesis and contributes to tumorigenesis.
Nature communications.
14(1)
[DOI] 10.1038/s41467-023-37501-0.
[PMID] 37045819.
2023
HIRA-mediated loading of histone variant H3.3 controls androgen-induced transcription by regulation of AR/BRD4 complex assembly at enhancers.
bioRxiv : the preprint server for biology.
[DOI] 10.1101/2023.05.08.536256.
[PMID] 37214820.
2023
HIRA-mediated loading of histone variant H3.3 controls androgen-induced transcription by regulation of AR/BRD4 complex assembly at enhancers.
Nucleic acids research.
51(19):10194-10217
[DOI] 10.1093/nar/gkad700.
[PMID] 37638746.
2022
Inhibition of Mps1 kinase enhances taxanes efficacy in castration resistant prostate cancer.
Cell death & disease.
13(10)
[DOI] 10.1038/s41419-022-05312-8.
[PMID] 36229449.
2022
βArrestin1 regulates glucocorticoid receptor mitogenic signaling in castration‐resistant prostate cancer
The Prostate.
82(7):816-825
[DOI] 10.1002/pros.24324.
2021
Nuclear βArrestin1 regulates androgen receptor function in castration resistant prostate cancer
Oncogene.
40(14):2610-2620
[DOI] 10.1038/s41388-021-01730-8.
2019
Arginine vasopressin receptor 1a is a therapeutic target for castration-resistant prostate cancer
Science Translational Medicine.
11(498)
[DOI] 10.1126/scitranslmed.aaw4636.
[PMID] 31243151.
2018
Inhibition of androgen receptor transactivation function by adenovirus type 12 E1A undermines prostate cancer cell survival.
The Prostate.
78(15):1140-1156
[DOI] 10.1002/pros.23689.
[PMID] 30009471.
2018
Uropathogenic Escherichia coli invades bladder epithelial cells by activating kinase networks in host cells.
The Journal of biological chemistry.
293(42):16518-16527
[DOI] 10.1074/jbc.RA118.003499.
[PMID] 30166343.
2018
β-Arrestin1 mediates hMENA expression and ovarian cancer metastasis.
Proceedings of the National Academy of Sciences of the United States of America.
115(12):2856-2858
[DOI] 10.1073/pnas.1802643115.
[PMID] 29514955.
2018
βArrestin2 Mediates Renal Cell Carcinoma Tumor Growth.
Scientific reports.
8(1)
[DOI] 10.1038/s41598-018-23212-w.
[PMID] 29559707.
2017
Prostaglandin E2 Receptor 4 Mediates Renal Cell Carcinoma Intravasation and Metastasis
Cancer Letters.
391:50-58
[DOI] 10.1016/j.caniet.2017.01.007.
2017
Prostaglandin E2 receptor 4 mediates renal cell carcinoma intravasation and metastasis.
Cancer letters.
391:50-58
[DOI] 10.1016/j.canlet.2017.01.007.
[PMID] 28104442.
2016
Biased α-adrenergic receptor and βarrestin signaling in a cell culture model of benign prostatic hyperplasia.
Biochemical and biophysical research communications.
471(1):41-6
[DOI] 10.1016/j.bbrc.2016.02.002.
[PMID] 26850854.
2016
Feedback regulation of G protein-coupled receptor signaling by GRKs and arrestins.
Seminars in cell & developmental biology.
50:95-104
[DOI] 10.1016/j.semcdb.2015.12.015.
[PMID] 26773211.
2015
Arrestin2 modulates androgen receptor activation.
Oncogene.
34(24):3144-51
[DOI] 10.1038/onc.2014.252.
[PMID] 25109335.
2015
The stress response neuropeptide CRF increases amyloid-β production by regulating γ-secretase activity.
The EMBO journal.
34(12):1674-86
[DOI] 10.15252/embj.201488795.
[PMID] 25964433.
2014
G protein-coupled receptor kinase GRK5 phosphorylates moesin and regulates metastasis in prostate cancer.
Cancer research.
74(13):3489-500
[DOI] 10.1158/0008-5472.CAN-13-2708.
[PMID] 24755472.
2014
Production of the Escherichia coli common pilus by uropathogenic E. coli is associated with adherence to HeLa and HTB-4 cells and invasion of mouse bladder urothelium.
PloS one.
9(7)
[DOI] 10.1371/journal.pone.0101200.
[PMID] 25036370.
2014
βArrestin1 regulates the guanine nucleotide exchange factor RasGRF2 expression and the small GTPase Rac-mediated formation of membrane protrusion and cell motility.
The Journal of biological chemistry.
289(19):13638-50
[DOI] 10.1074/jbc.M113.511360.
[PMID] 24692549.
2013
Expansion of CCR8(+) inflammatory myeloid cells in cancer patients with urothelial and renal carcinomas.
Clinical cancer research : an official journal of the American Association for Cancer Research.
19(7):1670-80
[DOI] 10.1158/1078-0432.CCR-12-2091.
[PMID] 23363815.
2013
Maintenance of androgen receptor inactivation by S-nitrosylation.
Cancer research.
73(22):6690-9
[DOI] 10.1158/0008-5472.CAN-13-1042.
[PMID] 24121486.
2013
PGE2 promotes renal carcinoma cell invasion through activated RalA.
Oncogene.
32(11):1408-15
[DOI] 10.1038/onc.2012.161.
[PMID] 22580611.
2013
Protein s-nitrosylation measurement.
Methods in enzymology.
522:409-25
[DOI] 10.1016/B978-0-12-407865-9.00019-4.
[PMID] 23374195.
2012
Acute activation of β2-adrenergic receptor regulates focal adhesions through βArrestin2- and p115RhoGEF protein-mediated activation of RhoA.
The Journal of biological chemistry.
287(23):18925-36
[DOI] 10.1074/jbc.M112.352260.
[PMID] 22500016.
2012
Dynamin2 S-nitrosylation regulates adenovirus type 5 infection of epithelial cells.
The Journal of general virology.
93(Pt 10):2109-2117
[DOI] 10.1099/vir.0.042713-0.
[PMID] 22791607.
2012
G-protein coupled receptor kinase 5 regulates prostate tumor growth.
The Journal of urology.
187(1):322-9
[DOI] 10.1016/j.juro.2011.09.049.
[PMID] 22099983.
2012
Rap1GAP regulates renal cell carcinoma invasion.
Cancer letters.
320(1):65-71
[DOI] 10.1016/j.canlet.2012.01.022.
[PMID] 22266190.
2012
S-nitrosylation-regulated GPCR signaling.
Biochimica et biophysica acta.
1820(6):743-51
[DOI] 10.1016/j.bbagen.2011.03.007.
[PMID] 21397660.
2011
Aberrant PGE₂ metabolism in bladder tumor microenvironment promotes immunosuppressive phenotype of tumor-infiltrating myeloid cells.
International immunopharmacology.
11(7):848-55
[DOI] 10.1016/j.intimp.2011.01.033.
[PMID] 21315786.
2011
Dynamin2- and endothelial nitric oxide synthase-regulated invasion of bladder epithelial cells by uropathogenic Escherichia coli.
The Journal of cell biology.
192(1):101-10
[DOI] 10.1083/jcb.201003027.
[PMID] 21220511.
2011
PGE2 promotes angiogenesis through EP4 and PKA Cγ pathway.
Blood.
118(19):5355-64
[DOI] 10.1182/blood-2011-04-350587.
[PMID] 21926356.
2011
Prostaglandin E2 regulates renal cell carcinoma invasion through the EP4 receptor-Rap GTPase signal transduction pathway.
The Journal of biological chemistry.
286(39):33954-62
[DOI] 10.1074/jbc.M110.187344.
[PMID] 21832044.
2010
Prostaglandin E2 promotes lung cancer cell migration via EP4-betaArrestin1-c-Src signalsome.
Molecular cancer research : MCR.
8(4):569-77
[DOI] 10.1158/1541-7786.MCR-09-0511.
[PMID] 20353998.
2009
Arf GTPase-activating protein AGAP2 regulates focal adhesion kinase activity and focal adhesion remodeling.
The Journal of biological chemistry.
284(20):13489-13496
[DOI] 10.1074/jbc.M900469200.
[PMID] 19318351.
2009
Identification of betaArrestin2 as a corepressor of androgen receptor signaling in prostate cancer.
Proceedings of the National Academy of Sciences of the United States of America.
106(23):9379-84
[DOI] 10.1073/pnas.0900258106.
[PMID] 19458261.
2008
2,2-bis(4-chlorophenyl)-1,1-dichloroethylene stimulates androgen independence in prostate cancer cells through combinatorial activation of mutant androgen receptor and mitogen-activated protein kinase pathways.
Molecular cancer research : MCR.
6(9):1507-20
[DOI] 10.1158/1541-7786.MCR-07-2166.
[PMID] 18819937.
2008
Androgens transduce the G alphas-mediated activation of protein kinase A in prostate cells.
Cancer research.
68(9):3225-31
[DOI] 10.1158/0008-5472.CAN-07-5026.
[PMID] 18451148.
2007
Agonist-stimulated reactive oxygen species formation regulates beta2-adrenergic receptor signal transduction.
Biochemical pharmacology.
74(1):64-73
[PMID] 17451656.
2007
Regulation of beta-adrenergic receptor signaling by S-nitrosylation of G-protein-coupled receptor kinase 2.
Cell.
129(3):511-22
[PMID] 17482545.
2006
A role for the G12 family of heterotrimeric G proteins in prostate cancer invasion.
The Journal of biological chemistry.
281(36):26483-90
[PMID] 16787920.
2006
Expression and function of lysophosphatidic acid LPA1 receptor in prostate cancer cells.
Endocrinology.
147(10):4883-92
[PMID] 16809448.
2006
Nitric oxide regulates endocytosis by S-nitrosylation of dynamin.
Proceedings of the National Academy of Sciences of the United States of America.
103(5):1295-300
[PMID] 16432212.
2006
The G12 family of heterotrimeric G proteins promotes breast cancer invasion and metastasis.
Proceedings of the National Academy of Sciences of the United States of America.
103(21):8173-8
[PMID] 16705036.
2005
Androgen receptor activation by G(s) signaling in prostate cancer cells.
The Journal of biological chemistry.
280(12):11583-9
[PMID] 15653681.
2004
G proteins in cancer: the prostate cancer paradigm.
Science's STKE : signal transduction knowledge environment.
2004(216)
[PMID] 14734786.
2004
Induction of human dendritic cell maturation using transfection with RNA encoding a dominant positive toll-like receptor 4.
Journal of immunology (Baltimore, Md. : 1950).
172(11):7162-8
[PMID] 15153540.
2004
Interleukin 6 mediates the lysophosphatidic acid-regulated cross-talk between stromal and epithelial prostate cancer cells.
The Journal of biological chemistry.
279(20):21154-9
[PMID] 15024019.
2004
Lysophosphatidic acid promotes survival of androgen-insensitive prostate cancer PC3 cells via activation of NF-kappaB.
The Prostate.
61(2):105-13
[PMID] 15305333.
2004
Requirement for G proteins in insulin-like growth factor-I-induced growth of prostate cells.
Journal of molecular endocrinology.
33(1):165-73
[PMID] 15291751.
2003
Bradykinin receptor subtype 1 expression and function in prostate cancer.
Cancer research.
63(9):2037-41
[PMID] 12727816.
2003
Regulation of epidermal growth factor receptor internalization by G protein-coupled receptors.
Biochemistry.
42(10):2887-94
[PMID] 12627954.
2003
Requirement for direct cross-talk between B1 and B2 kinin receptors for the proliferation of androgen-insensitive prostate cancer PC3 cells.
The Biochemical journal.
371(Pt 2):581-7
[PMID] 12534343.
2003
Targeting Gbetagamma signaling to inhibit prostate tumor formation and growth.
The Journal of biological chemistry.
278(39):37569-73
[PMID] 12869546.
2002
Defining G protein beta gamma specificity for effector recognition.
Methods in enzymology.
344:421-34
[PMID] 11771401.
2002
G protein-coupled receptors provide survival signals in prostate cancer.
Clinical prostate cancer.
1(3):177-81
[PMID] 15046693.
2002
Guanosine phosphate binding protein coupled receptors in prostate cancer: a review.
The Journal of urology.
167(3):1458-63
[PMID] 11832770.
2002
Lysophosphatidic acid-regulated mitogenic ERK signaling in androgen-insensitive prostate cancer PC-3 cells.
International journal of cancer.
102(6):572-9
[PMID] 12447997.
2002
Mitogenic action of LPA in prostate.
Biochimica et biophysica acta.
1582(1-3):265-9
[PMID] 12069837.
2002
Src-dependent tyrosine phosphorylation regulates dynamin self-assembly and ligand-induced endocytosis of the epidermal growth factor receptor.
The Journal of biological chemistry.
277(29):26642-51
[PMID] 12011079.
2001
Bradykinin induced mitogenesis of androgen independent prostate cancer cells.
The Journal of urology.
165(6 Pt 1):2121-5
[PMID] 11371939.
2000
Assembly of an A kinase-anchoring protein-beta(2)-adrenergic receptor complex facilitates receptor phosphorylation and signaling.
Current biology : CB.
10(7):409-12
[PMID] 10753752.
2000
Essential role for G proteins in prostate cancer cell growth and signaling.
The Journal of urology.
164(6):2162-7
[PMID] 11061948.
2000
Role of endocytosis in the activation of the extracellular signal-regulated kinase cascade by sequestering and nonsequestering G protein-coupled receptors.
Proceedings of the National Academy of Sciences of the United States of America.
97(4):1489-94
[PMID] 10677489.
2000
The beta(2)-adrenergic receptor mediates extracellular signal-regulated kinase activation via assembly of a multi-receptor complex with the epidermal growth factor receptor.
The Journal of biological chemistry.
275(13):9572-80
[PMID] 10734107.
1999
Beta-arrestin-dependent formation of beta2 adrenergic receptor-Src protein kinase complexes.
Science (New York, N.Y.).
283(5402):655-61
[PMID] 9924018.
1999
Delta opioid receptors expressed by stably transfected jurkat cells signal through the map kinase pathway in a ras-independent manner.
Journal of neuroimmunology.
94(1-2):48-57
[PMID] 10376935.
1999
Pleiotropic coupling of G protein-coupled receptors to the mitogen-activated protein kinase cascade. Role of focal adhesions and receptor tyrosine kinases.
The Journal of biological chemistry.
274(20):13978-84
[PMID] 10318809.
1999
Regulation of tyrosine kinase cascades by G-protein-coupled receptors.
Current opinion in cell biology.
11(2):177-83
[PMID] 10209148.
1999
Serotonin 5-HT1A receptor-mediated Erk activation requires calcium/calmodulin-dependent receptor endocytosis.
The Journal of biological chemistry.
274(8):4749-53
[PMID] 9988712.
1999
Src-mediated tyrosine phosphorylation of dynamin is required for beta2-adrenergic receptor internalization and mitogen-activated protein kinase signaling.
The Journal of biological chemistry.
274(3):1185-8
[PMID] 9880482.
1998
beta-arrestins regulate mitogenic signaling and clathrin-mediated endocytosis of the insulin-like growth factor I receptor.
The Journal of biological chemistry.
273(48):31640-3
[PMID] 9822622.
1998
Essential role for G protein-coupled receptor endocytosis in the activation of mitogen-activated protein kinase.
The Journal of biological chemistry.
273(2):685-8
[PMID] 9422717.
1998
Mechanisms of beta-adrenergic receptor desensitization and resensitization.
Advances in pharmacology (San Diego, Calif.).
42:416-20
[PMID] 9327928.
1998
Molecular basis for interactions of G protein betagamma subunits with effectors.
Science (New York, N.Y.).
280(5367):1271-4
[PMID] 9596582.
1998
The G protein-coupled receptor kinase 2 is a microtubule-associated protein kinase that phosphorylates tubulin.
The Journal of biological chemistry.
273(20):12316-24
[PMID] 9575184.
1997
Clathrin-mediated endocytosis of the beta-adrenergic receptor is regulated by phosphorylation/dephosphorylation of beta-arrestin1.
The Journal of biological chemistry.
272(49):31051-7
[PMID] 9388255.
1997
G protein-coupled receptors mediate two functionally distinct pathways of tyrosine phosphorylation in rat 1a fibroblasts. Shc phosphorylation and receptor endocytosis correlate with activation of Erk kinases.
The Journal of biological chemistry.
272(50):31648-56
[PMID] 9395506.
1997
Induction of interleukin-2 receptor alpha gene by delta9-tetrahydrocannabinol is mediated by nuclear factor kappaB and CB1 cannabinoid receptor.
DNA and cell biology.
16(3):301-9
[PMID] 9115639.
1997
Ras-dependent mitogen-activated protein kinase activation by G protein-coupled receptors. Convergence of Gi- and Gq-mediated pathways on calcium/calmodulin, Pyk2, and Src kinase.
The Journal of biological chemistry.
272(31):19125-32
[PMID] 9235901.
1997
Receptor and G betagamma isoform-specific interactions with G protein-coupled receptor kinases.
Proceedings of the National Academy of Sciences of the United States of America.
94(6):2180-5
[PMID] 9122168.
1997
Switching of the coupling of the beta2-adrenergic receptor to different G proteins by protein kinase A.
Nature.
390(6655):88-91
[PMID] 9363896.
1997
The role of sequestration in G protein-coupled receptor resensitization. Regulation of beta2-adrenergic receptor dephosphorylation by vesicular acidification.
The Journal of biological chemistry.
272(1):5-8
[PMID] 8995214.
1996
Cannabinoid receptor proteins are increased in Jurkat, human T-cell line after mitogen activation.
The Journal of pharmacology and experimental therapeutics.
276(2):776-83
[PMID] 8632350.
1995
delta 9-Tetrahydrocannabinol, cytokines, and immunity to Legionella pneumophila.
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.).
209(3):205-12
[PMID] 7777582.
1995
Expression of cannabinoid receptor mRNA in murine and human leukocytes.
Advances in experimental medicine and biology.
373:91-6
[PMID] 7545349.
1995
Marijuana, receptors and immunomodulation.
Advances in experimental medicine and biology.
373:103-13
[PMID] 7668140.
1994
delta 9-Tetrahydrocannabinol enhances the secretion of interleukin 1 from endotoxin-stimulated macrophages.
The Journal of pharmacology and experimental therapeutics.
270(3):1334-9
[PMID] 7932187.
1990
Target dependence of antisense oligodeoxynucleotide inhibition of c-Ha-ras p21 expression and focus formation in T24-transformed NIH3T3 cells.
Oncogene research.
5(4):267-75
[PMID] 2204019.
1989
Calcitonin, zinc, and testicular function.
Metabolism: clinical and experimental.
38(8):714-7
[PMID] 2761409.
Grants
Jul 2019
– Jun 2020
The American Society for Cell Biology Early Career Meeting Grant Application Requirements
Role: Principal Investigator
Funding: AMERICAN SOCIETY FOR CELL BIOLOGY
Sep 2018
– Aug 2020
A Novel Drug Target for Aggressive Prostate Cancer
Role: Principal Investigator
Funding: US DEPT OF VETERANS AFFAIRS
Jul 2015
– Jun 2024
Florida Academic Cancer Center Alliance Seed Grants
Role: Project Manager
Funding: UF HEALTH SHANDS HOSPITAL
Feb 2015
– Oct 2023
UF Health Cancer Center Pilot Project Grants funded through the Florida Consortium of National Cancer Institute Centers Program
Role: Project Manager
Funding: UF HEALTH SHANDS HOSPITAL
Jan 2015
– May 2024
UF Health Cancer Center Bridge Seed Grant funded through the Florida Consortium of National Cancer Institute Centers Program
Role: Project Manager
Funding: UF HEALTH SHANDS HOSPITAL
Sep 2014
– Aug 2019
Florida Minority Cancer Research and Training Center: Feasibility Studies
Role: Co-Investigator
Funding: NATL INST OF HLTH NCI
Aug 2014
– Aug 2016
The Florida Prostate Cancer Research Training Opportunities for Outstanding Leaders (ReTOOL) Program: Creating Opportunities for Minority HBCU Students
Role: Project Manager
Funding: US ARMY MED RES ACQUISITION
Mar 2010
– Feb 2016
Vesicle Trafficking and Bacteria Invasion
Role: Principal Investigator
Funding: NATL INST OF HLTH NIAID
Nov 2002
– Mar 2021
Haskell Hess Professorship/F004510
Role: Principal Investigator
Funding: UF FOUNDATION
Contact Details
Phones:
- Business:
- (352) 273-8112
Emails:
- Business:
- ydaaka@ufl.edu
Addresses:
- Business Mailing:
-
PO Box 103633
2033 MOWRY RD # 255
GAINESVILLE FL 32610